Novel 3D analysis using optical tissue clearing documents the evolution of murine rapidly progressive glomerulonephritis

Recent developments in optical tissue clearing have been difficult to apply for the morphometric analysis of organs with high cellular content and small functional structures, such as the kidney. Here, we establish combinations of genetic and immuno-labelling for single cell identification, tissue clearing and subsequent de-clarification for histoimmunopathology and transmission electron microscopy. Using advanced light microscopy and computational analyses, we investigated a murine model of crescentic nephritis, an inflammatory kidney disease typified by immune-mediated damage to glomeruli leading to the formation of hypercellular lesions and the rapid loss of kidney function induced by nephrotoxic serum.

Source: Kidney International - March 14, 2019 Category: Urology & Nephrology Authors: Victor G. Puelles, David Fleck, Lena Ortz, Stella Papadouri, Thiago Strieder, Alexander Boehner, James W. van der Wolde, Michael Vogt, Turgay Saritas, Christoph Kuppe, Astrid Fuss, Sylvia Menzel, Barbara M. Klinkhammer, Gerhard M üller-Newen, Felix Heyma Tags: Technical Notes Source Type: research