Tailoring Novel Soft Nano-vesicles ‘Flexosomes’ for Enhanced Transdermal Drug Delivery: Optimization, characterization and comprehensive ex vivo - in vivo evaluation

Publication date: Available online 10 February 2019Source: International Journal of PharmaceuticsAuthor(s): Hanaa A. Abdel-Messih, Rania A.H. Ishak, Ahmed S. Geneidi, Samar MansourAbstractThe transdermal route is a convenient non-invasive way for drug delivery, however, the hydrophobic compact nature of stratum corneum (SC) forms an obstacle hindering the diffusion of drugs particularly hydrophilic ones. Hence, the purpose of this study was to develop novel soft nano-vesicles, entitled Flexosomes, amalgamating two penetration enhancers, ethanol and one edge activator (EA) from various types and different hydrophilic-lipophilic balances. The tailored vesicles were loaded with tropisetron hydrochloride (TRO), a potent highly-soluble anti-emetic, and compared with ethosomes. Aiming to preclude the formation of rigid non-deformable mixed micelles, all critical parameters; EA type, phosphatidylcholine-to-EA molar ratio, and cholesterol concentration, were optimized proving their influences on vesicle-to-micelle transitions. The prepared formulations were characterized in terms of visual inspection, particle size, polydispersity, zeta potential, turbidity measurements, entrapment efficiency, and vesicle morphology. The permeation mechanisms were assessed by differential scanning calorimetry on isolated SC. The modified vesicles, based on ethanol and either vitamin E or PEGylated castor oil derivatives exhibited the highest transdermal fluxes confirmed by a deeply tracking to dermis...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research