LAMP-on-a-Chip: Revising Microfluidic Platforms for Loop-Mediated DNA Amplification

Publication date: Available online 31 January 2019Source: TrAC Trends in Analytical ChemistryAuthor(s): Haoqing Zhang, Ying Xu, Zdenka Fohlerova, Honglong Chang, Ciprian Iliescu, Pavel NeuzilAbstractNucleic acid amplification for the detection of infectious diseases, food pathogens, or assessment of genetic disorders require a laboratory setting with specialized equipment and technical expertise. Isothermal deoxyribonucleic acid amplification methods, such as loop-mediated isothermal amplification (LAMP), exhibit characteristics ideal for point-of-care (POC) applications, since their instrumentation is less demanding in comparison with the standard method of polymerase chain reaction. Other key advantages of LAMP are robustness and the production of pyrophosphate in the presence of the target gene, enabling to detect the reaction products using the naked eye. Polymerase inhibitors, presented in clinical samples, do not affect the amplification process, making LAMP suitable for a simple sample-to-answer diagnostic systems with simplified sample preparation. In this review, we discuss the trends in miniaturized LAMP techniques, such as microfluidic, paper-based, and digital with their advantages and disadvantages, especially for POC applications alongside our opinion of the future development of miniaturized LAMP.

Source: TrAC Trends in Analytical Chemistry - February 1, 2019 Category: Chemistry Source Type: research