Protection of Chickens Against Fowl Cholera by Supernatant Proteins of Pasteurella Multocida Cultured in an Iron- Restricted Medium.

Protection of Chickens Against Fowl Cholera by Supernatant Proteins of Pasteurella Multocida Cultured in an Iron- Restricted Medium.
Avian Pathol. 2019 Jan 14;:1-27
Authors: Luo Q, Kong L, Dong J, Zhang T, Wang H, Zhang R, Lu Q, Chen H, Shao H, Jin M
Abstract
Pasteurella multocida (P. multocida), a causative agent of fowl cholera, is an important pathogen in the poultry industry. In the present study, we found that the inactivated vaccine of P. multocida grown in an iron-restricted medium provided better protection than that grown in normal medium. Thus, we adopted a comparative proteomics approach, by using two-dimensional gel electrophoresis (2-DE), coupled with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/TOF MS), to profile the supernatant proteins associated with P. multocida under both conditions. Eleven upregulated proteins were identified, including aspartate ammonia-lyase (AspA), diacylglycerol kinase (DgK), 30S ribosomal protein S6 (RpsF), and eight outer membrane proteins (OMPs). To further characterize the three novel supernatant proteins identified under iron-restricted conditions, the AspA, DgK and RpsF proteins were expressed and purified, and used as immunogens to vaccinate chickens. The results showed that AspA, DgK and RpsF proteins developed 80.0%, 66.7%, and 80.0% immunity, respectively. These data indicated that the three novel proteins identified in the supernatant of t...

Source: Avian Pathology - January 14, 2019 Category: Pathology Authors: Luo Q, Kong L, Dong J, Zhang T, Wang H, Zhang R, Lu Q, Chen H, Shao H, Jin M Tags: Avian Pathol Source Type: research