Circulating Toll-like receptor 4-responsive microRNA panel in patients with coronary artery disease: Results from prospective and randomized study of treatment with renin-angiotensin system blockades

This study included 41 patients with CAD and 20 subjects without CAD (non-CAD).Plasma TLR4-responsive miRNA samples were analyzed using a microarray assay for 1,700 human miRNA. The candidate miRNAs were verified with real-time RT-PCR. Patients with CAD were randomized to 12 months of combined treatment with either telmisartan and atorvastatin (ARB) or enalapril and atorvastatin (ACEI). Plasma samples were obtained from peripheral blood at baseline and after 12 months. The microarray assay showed significant differences in 7 TLR4-responsive miRNAsbetween the CAD and non-CAD groups (P < 0.05). Real-time PCR verified that miR-31, miR-181a, miR-16 and miR-145 were significantly lower in the CAD group than in the non-CAD group (P < 0.01). Levels of TLR4 protein were higher in the CAD group than in the non-CAD group (P < 0.01) and were negatively correlated with levels of TLR4-responsive miRNAs. ROC curve analysis revealed that a panel of these four miRNAs was sensitive and specific enough to distinguish CAD from non-CAD (AUC = 0.93, 95%CI = 0.99-0.87). Both ARB and ACEI groups showed increased TLR4-responsive miRNAs and diminished levels of TLR4 protein (P < 0.05). Changes in miRNAs and TLR4 levels were greater in the ARB group than in the ACEI group(P < 0.05). Circulating TLR4-responsive miRNAs including miR-31, miR-181a, miR-16 and miR-145 ere significantly lower in patients with CAD compared to controls, and these miRNAs may be involved in the pathogenesis of CA...
Source: Clinical Science - Category: Biomedical Science Authors: Source Type: research