LncRNA TINCR / microRNA-107 / CD36 regulates cell proliferation and apoptosis in colorectal cancer via PPAR signaling pathway based on bioinformatics analysis.

LncRNA TINCR / microRNA-107 / CD36 regulates cell proliferation and apoptosis in colorectal cancer via PPAR signaling pathway based on bioinformatics analysis. Biol Chem. 2018 Dec 01;: Authors: Zhang X, Yao J, Shi H, Gao B, Zhang L Abstract Present study aims to determine the potential biomarkers and uncover the regulatory mechanisms of lncRNA TINCR / miR-107 / CD36 axis in CRC. Aberrantly expressed lncRNAs and differential-expressed genes were identified by analyzing the dataset GSE40967. Gene set enrichment analysis were employed, and Cytoscape software helps establishing the co-expression network between lncRNAs and genes. Quantitative RT-PCR analysis contributes to examining the expression levels of lncRNA TINCR, miR-107 and CD36. Dual luciferase assay was used to validate the association between miR-107 and lncRNA TINCR or CD36. EdU incorporation assay was employed, and flow cytometry was employed to detect cell apoptosis with Tumor Xenograft model utilized. Significantly dysregulated lncRNAs and mRNAs were identified. Peroxisome proliferators-activated receptor signaling pathway in CRC tissues was down-regulated. Loss of TINCR expression was associated with CRC progression. The expression levels of the TINCR and CD36 were down-regulated. We identified miR-107 as an inhibitory target of TINCR and CD36. Overexpression of TINCR could inhibit cell proliferation and promote apoptosis. MiR-107 overexpression in CRC cells induced prol...
Source: Biological Chemistry - Category: Chemistry Tags: Biol Chem Source Type: research