Ethyl acetate and n ‐butanol fraction of Cissus quadrangularis promotes the mineralization potential of murine pre‐osteoblast cell line MC3T3‐E1 (sub‐clone 4)

The present study reports the osteoblast differentiation and mineralization potential of ethyl acetate (CQ ‐EA) and butanol (CQ‐B) extracts ofCissus quadrangularis (CQ) on mouse pre ‐osteoblast cell line MC3T3‐E1 (sub‐clone 4) with an objective to isolate an antiosteoporotic compound. We have concluded in this part of the study that the CQ‐EA treatment resulted in early differentiation and mineralization as compared with the CQ‐B treatment. These findings suggest that low concentrations of CQ‐EA and CQ‐B have proliferative and osteogenic properties. CQ‐EA, however, is more potent osteogenic than CQ‐B. AbstractIn a sequel to investigate osteogenic potential of ethanolic extract ofCissus quadrangularis (CQ), the present study reports the osteoblast differentiation and mineralization potential of ethyl acetate (CQ ‐EA) and butanol (CQ‐B) extracts of CQ on mouse pre‐osteoblast cell line MC3T3‐E1 (sub‐clone 4) with an objective to isolate an antiosteoporotic compound. Growth curve, proliferation, and viability assays showed that both the extracts were nontoxic to the cells even at high concentration ( 100 µg/ml). The cell proliferation was enhanced at low concentrations (0.1 µg/ml and 1 µg/ml) of both the extracts. They also upregulated the osteoblast differentiation and mineralization processes in MC3T3‐E1 cells as reflected by expression profile of osteoblast marker genes such asRUNX2, Osterix, Collagen (COL1A1), Alkaline Phosphatase (A...
Source: Journal of Cellular Physiology - Category: Cytology Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research