Serotyping of dengue viruses circulating during 2014 –2015 in Assam, India

This study aimed to identify the dengue serotypes circulating in Assam. Clinically suspected dengue fever cases were included in the study. Viral RNA was extracted using QIAamp Viral RNA Mini Kit (Qiagen). Nested reverse transcriptase polymerase chain reaction was done for serotyping. The frequency of dengue was 25.23% with a peak during the period from September (22.56%) to October (26.22%). Two serotypes were detected, DEN-1 (72.7%) and DEN-2 (12.1%) and dual infection in 15.1%.
Source: Indian Journal of Medical Microbiology - Category: Microbiology Authors: Source Type: research

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Publication date: May 2019Source: Advances in Chronic Kidney Disease, Volume 26, Issue 3Author(s): Emmanuel A. BurdmannThe genus Flavivirus comprises approximately 73 viruses, which share several common aspects, such as dimension, structure, nucleic acid properties, and shape in electronic microscopy. Global incidence of flavivirus infection increased dramatically over the last decades, causing large outbreaks in several areas of the world. These viruses are expanding from endemic tropical and subtropical areas to previously nonendemic areas, affecting and causing diseases in millions of individuals worldwide and posing a ...
Source: Advances in Chronic Kidney Disease - Category: Urology & Nephrology Source Type: research
by Robert C. Reiner Jr., Steven T. Stoddard, Gonzalo M. Vazquez-Prokopec, Helvio Astete, T. Alex Perkins, Moises Sihuincha, Jeffrey D. Stancil, David L. Smith, Tadeuz J. Kochel, Eric S. Halsey, Uriel Kitron, Amy C. Morrison, Thomas W. Scott During the last 50 years, the geographic range of the mosquitoAedes aegypti has increased dramatically, in parallel with a sharp increase in the disease burden from the viruses it transmits, including Zika, chikungunya, and dengue. There is a growing consensus that vector control is essential to preventAedes-borne diseases, even as effective vaccines become available. What remains uncl...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
Mark K. Slifka1* and Ian J. Amanna2 1Division of Neuroscience, Oregon National Primate Research Center, Oregon Health &Science University, Beaverton, OR, United States2Najít Technologies, Inc., Beaverton, OR, United States Vaccines play a vital role in protecting our communities against infectious disease. Unfortunately, some vaccines provide only partial protection or in some cases vaccine-mediated immunity may wane rapidly, resulting in either increased susceptibility to that disease or a requirement for more booster vaccinations in order to maintain immunity above a protective level. The durability of a...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Viveros-Sandoval Reyes-Sandoval Chikungunya fever is a debilitating disease caused by Chikungunya virus (CHIKV) that can result in long-lasting arthralgias. The early diagnosis of CHIKV relies on PCR during the acute infection phase to allow differential diagnosis with other co-circulating arboviruses such as dengue and Zika. Alternatively, serology can support diagnosis and provide epidemiological information on current and past outbreaks. Many commercial serological ELISA assays are based on the inactivated whole CHIKV, but their sensitivity and specificity show great variability. We produced recombinant CHIKV E...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
Conclusion: Our results are similar to those reported from the state of Veracruz, Mexico, in which out of 33 samples of urine of patients with GBS two had a positive RT-PCR for ZIKV. Simultaneous processing of serum, CSF, urine, and saliva by RT-PCR may increase the success of diagnosis of GBS associated to ZIKV. Introduction In April 2016, a report of Epidemiological Surveillance for Zika virus (ZIKV) disease in Mexico reported 93 autochthonous laboratory-confirmed cases, collected between November 2015 and February 2016, and distributed amongst eight states of the country (1). In these patients, clinical manifest...
Source: Frontiers in Neurology - Category: Neurology Source Type: research
Abstract Lassa fever is an acute viral haemorrhagic illness caused by Lassa virus (LASV), which is endemic throughout much of West Africa. The virus primarily circulates in the Mastomys natalensis reservoir and is transmitted to humans through contact with infectious rodents or their secretions; human-to-human transmission is documented as well. With the exception of Dengue fever, LASV has the highest human impact of any haemorrhagic fever virus. On-going outbreaks in Nigeria have resulted in unprecedented mortality. Consequently, the World Health Organization (WHO) has listed LASV as a high priority pathogen for ...
Source: Immunology Letters - Category: Allergy & Immunology Authors: Tags: Immunol Lett Source Type: research
We describe the unexpected binding of S-layer to cells devoid of DC-SIGN but also confirm that the presence of DC-SIGN was essential for S-layer’s antiviral activity. S-layer protein exerted its antiviral effect with different kinetics than mannan, a known viral inhibitor that also acts on DC-SIGN (Yu et al., 2017). Together our results suggest that inhibition of viral entry by S-layer occurs via a novel S-layer/DC-SIGN interaction. Materials and Methods Isolation of S-Layer Proteins S-layer proteins were extracted from overnight cultures of L. acidophilus ATCC 4356 cells grown in MRS medium at 37°C by using 6...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, we exposed apically well-differentiated human NECs cultured at the ALI to the related flaviviruses ZIKV, JEV, WNV, and Usutu virus (USUV). We selected these viruses due to the recent increasing evidences of potential threat to humans (Cadar et al., 2017; Simonin et al., 2018). We show that NECs are particularly susceptible to JEV and WNV infection and to other flaviviruses included in this study. Infection with each virus led to shedding of infectious virus particles through the apical and basolateral surfaces and triggered host mechanisms at the level of inflammatory and antiviral mediators. Given...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
The Oropouche virus (OROV) is an arbovirus member of the Peribunyaviridae family and the Orthobunyavirus genus (Briese et al., 2016), responsible for a febrile syndrome that has affected people since its first discovery in 1955 in Trinidad and Tobago (Anderson et al., 1961). The etiological diagnosis of OROV infection is challenging due to the clinical similarities with other arboviral diseases including dengue, chikungunya, zika, and mayaro viruses, in addition to other pathogens such as Malaria (da Costa et al., 2017; Alvarez-Falconi and R íos, 2010; Travassos da Rosa et al., 2017), and the lack of molecular diffe...
Source: International Journal of Infectious Diseases - Category: Infectious Diseases Authors: Source Type: research
In this study, we evaluated ATA as a potential antiviral drug against ZIKV replication. The antiviral activity of ATA against ZIKV replication in vitro showed median inhibitory concentrations (IC50) of 13.87 ± 1.09 μM and 33.33 ± 1.13 μM in Vero and A549 cells, respectively; without showing any cytotoxic effect in both cell lines (median cytotoxic concentration (CC50)> 1,000 μM). Moreover, ATA protected both cell types from ZIKV-induced cytopathic effect (CPE) and apoptosis in a time- and concentration-dependent manner. In addition, pre-treatment of Vero cells with ATA for up to 72 h also resulted...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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