A gentle approach to investigate the influence of LRP-1 silencing on the migratory behavior of breast cancer cells by atomic force microscopy and dynamic cell studies

Publication date: Available online 10 November 2018Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Alexandre Berquand, Marie Meunier, Jessica Thevenard Devy, Corinne Ivaldi, Océane Campion, Stéphane Dedieu, Michael Molinari, Jérôme DevyAbstractTo get more insight into the role of LRP-1 in the mechanism of tumor progression in triple negative breast cancer. Atomic force microscopy, videomicroscopy, confocal microscopy and Rho-GTPAse activity assay were used on MDA-MB-231 and LRP-1-silenced cells. Silencing of LRP-1 in MDA-MB-231 cells was shown to led to a dramatic increase in the Young's modulus in parallel to a spectacular drop in membrane extension dynamics as well as a decrease in the cells migration abilities on both collagen I and fibronectin substrates. These results were perfectly correlated to a corresponding change in cell morphology and spreading capacity as well as in Rho-GTPases activity. By a multi-technique approach, it was demonstrated that LRP-1 played a crucial role in the migration of MDA-MB-231 cells by modulating the membrane extension dynamic. The originality of this AFM investigation lies in the non-invasive aspect of the measurements.Graphical AbstractMDA-MB-231 shCtrl and shLRP-1 have been characterized by using a conical indentor model in order to obtain Young's modulus images of live cells on and correlated to migration abilities.
Source: Nanomedicine: Nanotechnology, Biology and Medicine - Category: Nanotechnology Source Type: research