Generation of induced pluripotent stem cells from a patient with hearing loss carrying GJB2 p.V37I mutation

Publication date: Available online 3 October 2018Source: Stem Cell ResearchAuthor(s): Yen-Hui Chan, Yen-Fu Cheng, You-Tzung Chen, Cheng-Yen Huang, Chin-Hsien Lin, Chin-Ju Hu, Ying-Chang Lu, Chen-Chi Wu, Chuan-Jen HsuAbstractRecessive mutations in the GJB2 gene are the most common genetic cause of hearing loss in humans. By using the Sendai-virus delivery system, we generated induced pluripotent stem cells (iPSCs) from the peripheral blood mononuclear cells of a female patient with the p.V37I (c.109G > A) mutation, a GJB2 mutation highly prevalent in the Asian population. The resulting iPSCs had a normal karyotype. The iPSCs also showed pluripotency, as confirmed by immunofluorescence staining, and differentiated into the three germ layers in vivo. This cellular model will provide a useful platform for investigating the pathogenic mechanisms of deafness related to GJB2 mutations.
Source: Stem Cell Research - Category: Stem Cells Source Type: research