Chemical proteomics for subcellular proteome analysis.

Chemical proteomics for subcellular proteome analysis.
Curr Opin Chem Biol. 2018 Aug 28;48:1-7
Authors: Zhu H, Tamura T, Hamachi I
Abstract
Protein functions are tightly regulated by their subcellular localization and dynamic alteration. Chemical proteomics offers convenience and efficiency for profiling protein features in a native context. In this review, we summarize the recent progress of subcellular-compartment-focused chemical proteomics which do not rely on organelle fractionation. Organelle-specific activity-based protein profiling (ABPP) and engineered ascorbate peroxidase (APEX) have been developed for proteome analysis within organelles and even sub-organelles. In parallel, our lab designed organelle-localizable reactive molecules (ORMs) to selectively tag nuclear and mitochondrial proteins. ORMs-based proteomics is applicable to primary neurons and brain slices, as well as cultured cell lines. In addition, we invented a conditional proteomics approach to elucidate zinc homeostasis by labeling and identifying proteins localized in Zn2+-rich space of live cells.
PMID: 30170243 [PubMed - as supplied by publisher]

Source: Current Opinion in Chemical Biology - August 28, 2018 Category: Biochemistry Authors: Zhu H, Tamura T, Hamachi I Tags: Curr Opin Chem Biol Source Type: research