Acute decompression following simulated dive conditions alters mitochondrial respiration and motility.

Acute decompression following simulated dive conditions alters mitochondrial respiration and motility. Am J Physiol Cell Physiol. 2018 Aug 15;: Authors: Jang DH, Owiredu S, Ranganathan A, Eckmann DM Abstract While barotrauma, decompression sickness and drowning-related injuries are common morbidities associated with diving and decompression from depth, it remains unclear what impact rapid decompression has on mitochondrial function. In vitro diving simulation was performed with human dermal fibroblast cells subjected to control, air, nitrogen and oxygen dive conditions. With the exception of gas mixture, all other related variables including absolute pressure exposure, dive and decompression rates, and temperature were held constant. High-resolution respirometry was used to examine key respiratory states. Mitochondrial dynamic function including net movement, number, and rates of fusion/fission events were obtained from fluorescence microscopy imaging. Effects of the dive conditions on cell cytoskeleton were assessed by imaging both actin and microtubules. Fibroblasts in the Air group had lower Max respiration when compared to both the Control and Nitrogen groups. The Oxygen group had overall lower respiration when compared to all other groups. All groups demonstrated lower mitochondrial motility when compared to the Control group. Rates of fusion and fission events were the same between all groups. There were visible differences in ...
Source: Am J Physiol Cell Ph... - Category: Cytology Authors: Tags: Am J Physiol Cell Physiol Source Type: research