TLR2 Ligand Pam3CSK4 Regulates MMP-2/9 Expression by MAPK/NF- κB Signaling Pathways in Primary Brain Microvascular Endothelial Cells.

This study showed that Pam3CSK4 upregulated the mRNA and protein expression of MMP-9 and downregulated MMP-2 and TJ expression in BMECs simultaneously. Pam3CSK4 also induced the phosphorylation of MAPKs and NF-κB signaling pathways in BMECs. MMP-9 expression was found to decrease by pretreatment with inhibitors of ERK1/2 and JNK but not p38. However, the mRNA and protein expression of MMP-2 and MMP-9 increased after addition of a NF-κB inhibitor. Our results indicated that Pam3CSK4 was able to upregulate MMP-9 expression through ERK1/2 and JNK signaling pathways, but the NF-κB signaling pathway negatively regulated the effect of TLR2 on MMP-2 and MMP-9 expression in BMECs. The finding provides novel insight into the molecular mechanism of MMP-2/9 expression in BMECs.
PMID: 30088235 [PubMed - as supplied by publisher]

Source: Neurochemical Research - August 7, 2018 Category: Neuroscience Authors: Zhu H, Dai R, Zhou Y, Fu H, Meng Q Tags: Neurochem Res Source Type: research