Synergistic interactions of Angiotensin Converting Enzyme (ACE) gene and Apolipoprotein E (APOE) gene polymorphisms with T1DM susceptibility in south India

Publication date: Available online 25 July 2018Source: Meta GeneAuthor(s): Padma-Malini Ravi, Rathika Chinniah, Ramgopal Sivanadham, Murali Vijayan, Dharmarajan Pannerselvam, S. Pushkala, Balakrishnan KaruppiahAbstractType 1 diabetes mellitus (T1DM) is an autoimmune endocrine disease, in which both gene-gene and gene-environmental factors play a key role in the development and progression of the disease. The main aim of the present study was to determine the association of Angiotensin Converting Enzyme (ACE) and Apolipoprotein E (APOE) gene polymorphisms in T1DM patients. A cohort of 196 T1DM patients (n = 196) and age/sex matched controls (n = 196) were enrolled for typing of ACE and APOE by PCR-SSP and PCR-RFLP methods. We observed an increased frequencies of ‘DD’ (P: OR = 2.40; M: OR = 2.08 and F: OR = 2.90), ‘ID’ (P: OR = 1.94; M: OR = 2.01; F: OR = 1.84) genotypes and ‘D’ allele (P: OR = 2.43; M: OR = 2.21; F: OR = 2.74) in patients confirms their susceptible association. Whereas, the ‘II’ genotype (P: OR = 0.25; M: OR = 0.28; F: OR = 0.21) and ‘I’ allele frequencies (P: OR = 0.41; M: OR = 0.45; F: OR = 0.36) were decreased in patients suggested their protective association. Multivariate analysis revealed that the ‘GAD65+’, ‘IA2+’ and ‘GAD65+ & IA2+’ autoantibodies were significantly associated with patients. Genetic model of inheritance analysis showed that, the additive ...
Source: Meta Gene - Category: Genetics & Stem Cells Source Type: research