Production of glutaric acid from 5-aminovaleric acid using Escherichia coli whole cell bio-catalyst overexpressing GabTD from Bacillus subtilis

In this study, as a solution to improve the production of glutaric acid, we introduced gabTD genes from B. subtilis to E. coli for a whole cell biocatalytic approach. This approach enabled us to determine the effect of co-factors on reaction and to achieve a high conversion yield from 5-aminovaleric acid at the optimized reaction condition. Optimization of whole cell reaction by different plasmids, pH, temperature, substrate concentration, and cofactor concentration achieved full conversion with 100 mM of 5-aminovaleric acid to glutaric acid. Nicotinamide adenine dinucleotide phosphate (NAD(P)+) and α-ketoglutaric acid were found to be critical factors in the enhancement of conversion in selected conditions. Whole cell reaction with a higher concentration of substrates gave 141 mM of glutaric acid from 300 mM 5-aminovaleric acid, 150 mM α-ketoglutaric acid, and 60 mM NAD+ at 30 °C, with a pH of 8.5 within 24 hours (47.1% and 94.2% of conversion based on 5-aminovaleric acid and α-ketoglutaric acid, respectively). The whole cell biocatalyst was recycled 5 times with the addition of substrates; this enabled the accumulation of extra glutaric acid.
Source: Enzyme and Microbial Technology - Category: Biotechnology Source Type: research

Related Links:

Publication date: Available online 18 May 2019Source: Journal of Global Antimicrobial ResistanceAuthor(s): Giuseppe Vittorio De Socio, Paola Rubbioni, Daniele Botta, Elio Cenci, Alessandra Belati, Riccardo Paggi, Maria Bruna Pasticci, Antonella MencacciAbstractObjectivesThe aim of the study was to evaluate a cumulative antimicrobial resistance index (ARI) as a possible key outcome measure of antimicrobial stewardship (AS) programs and as a tool to predict antimicrobial resistance (AMR) trend.MethodsAntibiotic susceptibility for Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pse...
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
ConclusionOnly 33.3% of the hemolyticUPEC isolates exhibited the phenotypic and genotypic features of Cnf 1 and Colibactin. The in vivo animal model results gives an evidence of active Cnf 1 and Colibactin expression and indicates the risks associated with recurrent and chronic UTIs caused byUPEC. The bioinformatics analyses confirmed the predominance of colibactinpks island among Enterobacteriaceae family (92.86%), with the highest occurrence amongEscherichia species (53.57%), followed byKlebsiella (28.57%),Citrobacter (7.14%), andEnterobacter species (3.57%). The Cnf 1 is predominant amongEscherichia coli (94.05%) and sp...
Source: Gut Pathogens - Category: Microbiology Source Type: research
Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): Juliana B. Coitinho, Mariana A.F. Costa, Eliza M. Melo, Elis A. Morais, Lorena G.A. de Andrade, Aline M. da Rocha, Mariana T.Q. de Magalhães, Denize C. Favaro, Lucas Bleicher, Enio R.P. Pedroso, Alfredo M. Goes, Ronaldo A.P. NagemAbstractPb27 antigen is an interesting alternative to immunological diagnosis of Paracoccidioidomycosis (PCM) and has demonstrated to be protective in experimental PCM. Its tertiary structure and possible function remained unknown till now. To study Pb27 at the atomic level, the recombinant protein was expressed...
Source: Molecular Immunology - Category: Allergy & Immunology Source Type: research
Publication date: Available online 18 May 2019Source: Materials Science and Engineering: CAuthor(s): Nur Arifah Ismail, Khairul Anuar Mat Amin, Fadzillah @. Adibah Abdul Majid, Mohd Hasmizam RazaliAbstractIn this work, the potential of titanium dioxide nanoparticles incorporated gellan gum (GG + TiO2-NPs) biofilm as wound dressing material was investigated. The GG + TiO2-NPs biofilm was prepared via evaporative casting technique and was characterized using FTIR, XRD, and SEM to study their physiochemical properties. The mechanical properties, swelling and water vapor transmission rate (WVTR) of biofilm was determin...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Publication date: Available online 16 May 2019Source: Food ChemistryAuthor(s): Emma Talón, Anna-Maija Lampi, María Vargas, Amparo Chiralt, Kirsi Jouppila, Chelo González-MartínezAbstractThe encapsulation of eugenol (E) by spray-drying using whey protein (WP) or soy lecithin (LE) and maltodextrin in combination with oleic acid (OA) and chitosan (CH) was analysed in order to obtain antioxidant and antimicrobial powders for food applications. Formulations with only WP or LE showed higher encapsulation efficiencies (EE) (95-98%) and antibacterial effect against E. coli and L. innocua due to their gr...
Source: Food Chemistry - Category: Food Science Source Type: research
In this study, we employed a multipurpose fusion tag to improve the production of PfPMV in E. coli. Recombinant PfPMVm, comprising residues 84 to 521, was substantially obtained in soluble form and could be purified in a single step, yielding a 3.7-fold increase in purified PfPMVm compared to previous reports. Additionally, we have mutated the catalytic residues (D118 N and D365 N), individually and together, and the unpaired cysteine residue C178 to evaluate the effects on catalytic efficiency. Mutation of D365 had more pronounced effects on the catalytic efficiency than that of D118, suggesting that the D36...
Source: Molecular and Biochemical Parasitology - Category: Parasitology Source Type: research
ConclusionsHand hygiene and an intensive isolation strategy remained the most critical precautions for preventing an outbreak of healthcare-associated viral gastroenteritis in the neonate care unit.
Source: Journal of Microbiology, Immunology and Infection - Category: Microbiology Source Type: research
This study was the first to identify and characterize NaCl-tolerant β-xylosidase/α-l-arabinofuranosidase from the gut microbiota. The enzyme's salt, xylose, and alkali stability and resistance to various chemicals make it a potential biocatalyst for the saccharification of lignocellulose, the food industry, and industrial processes conducted in sea water.
Source: Journal of Bioscience and Bioengineering - Category: Biomedical Science Source Type: research
Publication date: Available online 18 May 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Sabrina Belbekhouche, Noureddine Bousserrhine, Vanessa Alphonse, Fannie Le Floch, Youcef Charif Mechiche, Ilyes Menidjel, Benjamin CarbonnierAbstractCreating an appropriate antibacterial disinfection system without forming any harmful compounds is still a major challenge and calls for new technologies for efficient disinfection and microbial control. Towards this aim, we report on the elaboration of biodegradable and biocompatible polymeric nanocapsules, also called hollow nanoparticles, for potential applications in anti...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
Publication date: Available online 17 May 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Massimiliano Magro, Davide Baratella, Simone Molinari, Andrea Venerando, Gabriella Salviulo, Giulia Chemello, Ike Olivotto, Giorgio Zoppellaro, Juri Ugolotti, Claudia Aparicio, Jiri Tucek, Anna P. Fifi, Giuseppe Radaelli, Radek Zboril, Fabio VianelloAbstractA category of naked maghemite nanoparticles (γ-Fe2O3), named surface active maghemite nanoparticles (SAMNs), is characterized by biological safety, high water colloidal stability and a surface chemistry permitting the binding of ligands. In the present study, the...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
More News: Biotechnology | Chemistry | Gastroenteritis | Genetics | Study