Characterization of modified mesoporous silica nanoparticles as vectors for siRNA delivery

Publication date: Available online 21 February 2018Source: Asian Journal of Pharmaceutical SciencesAuthor(s): Anna Slita, Anna Egorova, Eudald Casals, Anton Kiselev, Jessica M. RosenholmAbstractGene therapy using siRNA molecules is nowadays considered as a promising approach. For successful therapy, development of a stable and reliable vector for siRNA is crucial. Non-viral and non-organic vectors like mesoporous silica nanoparticles (MSN) are associated with lack of most viral vector drawbacks, such as toxicity, immunogenicity, but also generally a low nucleic acid carrying capacity. To overcome this hurdle, we here modified the pore walls of MSNs with surface-hyperbranching polymerized poly(ethyleneimine) (hbPEI), which provides an abundance of amino-groups for loading of a larger amount of siRNA molecules via electrostatic adsorption. After loading, the particles were covered with a second layer of pre-polymerized PEI to provide better protection of siRNA inside the pores, more effective cellular uptake and endosomal escape. To test the transfection efficiency of PEI covered siRNA/MSNs, MDA-MB 231 breast cancer cells stably expressing GFP were used. We demonstrate that PEI-coated siRNA/MSN complexes provide more effective delivery of siRNAs compared to unmodified MSNs. Thus, it can be concluded that appropriately surface-modified MSNs can be considered as prospective vectors for therapeutic siRNA delivery.Graphical abstractModified mesoporous silica nanoparticles (MSNs) wh...
Source: Asian Journal of Pharmaceutical Sciences - Category: Drugs & Pharmacology Source Type: research

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Conclusions: We found that several conserved tumor stromal genes might regulate breast cancer invasion through ECM remodeling. The clinical outcome analyses of COL1A1 and FN1 suggest these two genes are promising targets for future studies. PMID: 30237810 [PubMed - in process]
Source: Cellular and Molecular Biology Letters - Category: Biochemistry Authors: Tags: Cell Mol Biol Lett Source Type: research
Publication date: October 2018Source: Phytochemistry Letters, Volume 27Author(s): Qiao-Ling Hu, Pei-Qian Wu, Ying-Hong Liu, Feng-Ming Qi, Chun-Xue Yu, Ye Zhao, Yi-Fan Yu, Dong-Qing Fei, Zhan-Xin ZhangAbstractThree new sesquiterpene lactones, 5α-hydroxy-4α,15-epoxy-11αH-eudesman-12,8β-olide (1), carabrol-4-O-palmitate (2) and carabrol-4-O-linoleate (3), along with five known ones, were isolated from the whole plant of Carpesium abrotanoides. The structures of new compounds were determined by extensive spectroscopic analysis, and compound 1 was also elucidated by X-ray single-crystal diffraction analys...
Source: Phytochemistry Letters - Category: Chemistry Source Type: research
Publication date: October 2018Source: Phytochemistry Letters, Volume 27Author(s): Moustafa T. Gabr, Mohammed S. Abdel-RaziqAbstractNew series of arylsparteine derivatives were synthesized and evaluated for their cytotoxic activity against four human cancer cell lines (cervical epithelial carcinoma cells Hela, breast cancer cells MCF-7, lung cancer cells A549, and glioma cells U87 MG) and one normal fibroblast cell line. Structure-activity relationship revealed that introduction of 4-quinolinyl moiety to sparteine afforded a hybrid compound 10 with considerable antiproliferative activity against all tested cancer cell lines...
Source: Phytochemistry Letters - Category: Chemistry Source Type: research
Publication date: Available online 22 September 2018Source: Diagnostic and Interventional ImagingAuthor(s): R. Rella, E. Bufi, P. Belli, A. Contegiacomo, M. Giuliani, M. Rosignuolo, P. Rinaldi, R. ManfrediAbstractBackground parenchymal enhancement (BPE) on breast magnetic resonance imaging (MRI) is a dynamic process, which varies among women and within the same woman over time due to different factors. BPE has profound implications for women with or at risk of breast cancer. Breast radiologist should be aware of factors that could potentially influence BPE and have to be familiar with its typical appearance. Marked BPE cou...
Source: Diagnostic and Interventional Imaging - Category: Radiology Source Type: research
This article examines current literature on male breast cancer, including reasons for the gender disparity and interventions that may help to address it. METHODS: An integrative review was conducted, and 12 studies were selected for inclusion. FINDINGS: Study consensus is that male breast cancer is an underresearched subject and that increased research efforts may improve outcomes. In addition, interventions could help to decrease the gender disparity in breast cancer detection. PMID: 30239521 [PubMed - in process]
Source: Clinical Journal of Oncology Nursing - Category: Nursing Authors: Tags: Clin J Oncol Nurs Source Type: research
This article describes the experience of a large multisite organization that implemented a machine-based scalp cooling program. METHODS: Nursing staff led an interprofessional team that addressed components of the program. As a result, eight sites within the authors' organization simultaneously began offering scalp cooling via machine using a single unified process. This approach was then successfully replicated one year later to prepare six additional sites to launch scalp cooling for other solid tumor types beyond breast cancer. FINDINGS: Using a structured, collaborative, and interprofessional approach to the ...
Source: Clinical Journal of Oncology Nursing - Category: Nursing Authors: Tags: Clin J Oncol Nurs Source Type: research
Publication date: October 2018Source: Canadian Journal of Cardiology, Volume 34, Issue 10, Supplement 1Author(s): M. Altaha, M. Nolan, E. Amir, K. Connelly, C. Brezden-Masley, K. Yared, B. Wintersperger, P. Thavendiranathan
Source: Canadian Journal of Cardiology - Category: Cardiology Source Type: research
ConclusionScreening for breast cancer may help diagnose breast cancer at an early stage. Women with previous cancer who are not undergoing current treatment or in palliative care have the same propensity to participate as other women invited to breast cancer screening. Women with previous gynaecological cancer were less likely to participate in breast cancer screening than women with other cancer types. These results may only be generalised to similar health care systems.
Source: Journal of Cancer Research and Clinical Oncology - Category: Cancer & Oncology Source Type: research
Publication date: Available online 21 September 2018Source: Journal of Molecular StructureAuthor(s): Israel González-Méndez, José D. Solano, Pasquale Porcu, Andrea Ruiu, Yareli Rojas-Aguirre, Ernesto RiveraAbstractDoxorubicin(Dox) is a potent anti-cancer drug used in the treatment of triple negative breast cancer (TNBC), however, it causes dose-dependent cardiotoxicity once the treatment is concluded. One of the new strategies to overcome this problem is the design of pH sensitive prodrugs, which can improve its selectivity and reduce the toxic effects of the free drug. For that reason, the synthesis o...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
(Natural News) When you think of breast cancer, surgery and chemotherapy probably come immediately to mind. Many patients often end up undergoing one or both of these treatments, and the prospect of going under the knife or being injected with chemicals can be scarier to some people than the diagnosis itself. However, some patients are...
Source: - Category: Consumer Health News Source Type: news
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