Investigating Nrf2-associated non-coding RNAs in the hibernating ground squirrel, Ictidomys tridecemlineatus

Publication date: July 2018Source: Journal of Thermal Biology, Volume 75Author(s): Jacques J. Frigault, Josée D. Gaudet, Pier Jr MorinAbstractSmall mammals hibernate to deal with environmental conditions associated with the winter season. Numerous physiological changes occur during a typical torpor-arousal cycle including variations in heart rate and blood flow. Such cycle possesses characteristics of ischemia-reperfusion cycles that can lead to oxidative stress in non-hibernating models. Interestingly, hibernators can cope with these conditions and the complete molecular picture underlying this adaptation is not fully understood. Non-coding RNAs, including microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), can impact expression and activity of various targets and have been associated with oxidative stress response. This work was aimed at assessing expression of oxidative stress-associated non-coding RNAs and their targets during hibernation. Measurement of miRNAs miR-93, miR-141, miR-144 and miR-200a, lncRNAs Mhrt and ODRUL, as well as of several targets associated with the Nrf2 signaling cascade including Keap1 was conducted using qRT-PCR in hibernating hearts of the thirteen-lined ground squirrel, Ictidomys tridecemlineatus. Elevated Nrf2 levels and reduced miR-200a levels were notably observed in hibernating versus euthermic samples. Functional analysis of targets predicted to be regulated by the investigated miRNAs was performed and revealed transcriptional regulati...
Source: Journal of Thermal Biology - Category: Biology Source Type: research