Interaction of anthraquinones of Cassia occidentalis seeds with DNA and Glutathione

Publication date: 2018Source: Toxicology Reports, Volume 5Author(s): Gati Krushna Panigrahi, Neeraj Verma, Nivedita Singh, Somya Asthana, Shailendra K. Gupta, Anurag Tripathi, Mukul DasAbstractConsumption of Cassia occidentalis (CO) seeds has been associated with the hepatomyoencephalopathy (HME) in children. Recently, we have characterized the toxic anthraquinones (AQs) such as Emodin, Rhein, Aloe-emodin, Chrysophanol and Physcion in CO seeds and detected these moieties in the bio fluids of CO poisoning cases. As AQs were detected in the serum of HME patients, their interaction with key biomolecules including protein, DNA and glutathione (GSH) is imperative. In this regard, we have previously reported the interaction of these AQs with serum albumin protein and their subsequent biological effects. However, the interaction of these AQs with DNA and GSH remained unexplored. In the present work, we have studied the binding of these AQs of CO seeds with DNA and GSH by fluorescence spectroscopy, UV–vis spectral analysis, molecular docking, and biochemical studies. Results indicated a higher binding affinity for Emodin (Ka = 3.854 × 104 L mol−1 S−1), Aloe-emodin (Ka = 0.961 × 104 L mol−1 S−1) and Rhein (Ka = 0.034 × 104 L mol−1 S−1) towards calf thymus DNA may be associated with their higher cytotoxicity. Alternatively, Physcion and Chrysophanol which showed less cytotoxicity in our earlier studies exhibited very low DNA bi...

Source: Toxicology Reports - July 5, 2018 Category: Toxicology Source Type: research