A method for single-neuron chronic recording from the retina in awake mice

We report a nonsurgical method to achieve chronically stable in vivo recordings from single retinal ganglion cells (RGCs) in awake mice. We developed a noncoaxial intravitreal injection scheme in which injected mesh electronics unrolls inside the eye and conformally coats the highly curved retina without compromising normal eye functions. The method allows 16-channel recordings from multiple types of RGCs with stable responses to visual stimuli for at least 2 weeks, and reveals circadian rhythms in RGC responses over multiple day/night cycles.

Source: ScienceNOW - June 28, 2018 Category: Science Authors: Hong, G., Fu, T.-M., Qiao, M., Viveros, R. D., Yang, X., Zhou, T., Lee, J. M., Park, H.-G., Sanes, J. R., Lieber, C. M. Tags: Neuroscience, Techniques reports Source Type: news