Cancers, Vol. 10, Pages 206: Constitutive Activation of STAT3 in Myeloma Cells Cultured in a Three-Dimensional, Reconstructed Bone Marrow Model
Cancers, Vol. 10, Pages 206: Constitutive Activation of STAT3 in Myeloma Cells Cultured in a Three-Dimensional, Reconstructed Bone Marrow Model
Cancers doi: 10.3390/cancers10060206
Authors:
Yung-Hsing Huang
Ommoleila Molavi
Abdulraheem Alshareef
Moinul Haque
Qian Wang
Michael P. Chu
Christopher P. Venner
Irwindeep Sandhu
Anthea C. Peters
Afsaneh Lavasanifar
Raymond Lai
Malignant cells cultured in three-dimensional (3D) models have been found to be phenotypically and biochemically different from their counterparts cultured conventionally. Since most of these studies employed solid tumor types, how 3D culture affects multiple myeloma (MM) cells is not well understood. Here, we compared MM cells (U266 and RPMI8226) in a 3D culture model with those in conventional culture. While the conventionally cultured cells were present in single cells or small clusters, MM-3D cells grew in large spheroids. We discovered that STAT3 was the pathway that was more activated in 3D in both cell lines. The active form of STAT3 (phospho-STAT3 or pSTAT3), which was absent in MM cells cultured conventionally, became detectable after 1–2 days in 3D culture. This elevated pSTAT3 level was dependent on the 3D environment, since it disappeared after transferring to conventional culture. STAT3 inhibition using a pharmacological agent, Stattic, significantly decreased the cell viability of MM cells and sensitized them to bortezomib in 3D culture. Using an oligonucl...
Source: Cancers - Category: Cancer & Oncology Authors: Yung-Hsing Huang Ommoleila Molavi Abdulraheem Alshareef Moinul Haque Qian Wang Michael P. Chu Christopher P. Venner Irwindeep Sandhu Anthea C. Peters Afsaneh Lavasanifar Raymond Lai Tags: Article Source Type: research