A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci

CONCLUSION: The 16S-PCR-HRMA approach has the potential for detecting vancomycin-resistantE faeciumandE faecalis. Data with VRE provide the basis for combining VRE identification with pathogens speciation in a rapid, cheap assay able to identify a pathogen as an Enterococcus and whether it is vancomycin-sensitive or -resistantE faecium orE faecalis in a single PCR and HRMA run. LIMITATIONS: Tested on specific, but not all, referenceEnterococcus species and clinical isolates.  CONFLICT OF INTEREST:None. 
Source: Annals of Saudi Medicine - Category: General Medicine Tags: ISSUE 3 Source Type: research