Identification of Malassezia species by MALDI-TOF MS after expansion of database

The taxonomy of Malassezia species is evolving with introduction of molecular techniques, and difficulty is faced to identify the species by phenotypic methods. Among 15 known Malassezia species, the present Bruker database could identify only 2 species. The present study was aimed to improve Matrix Associated Laser Desorption Ionization -Time of Flight Mass Spectrometry (MALDI-TOF MS) based identification of Malassezia species. A total of 88 isolates (DNA sequencing confirmed) for database preparation and for the validation of database, 190 isolates confirmed by Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) were used.

Source: Diagnostic Microbiology and Infectious Disease - May 29, 2018 Category: Microbiology Authors: P Honnavar, A K Ghosh, S Paul, S A Shankarnarayan, P Singh, S Dogra, A Chakrabarti, S M Rudramurthy Source Type: research