Nonspecific amplification of human DNA by Streptococcus pneumoniae LytA primer

Conclusion: Since HRMA is done without a specific probe, one should be aware of non-specific amplifications while using primers for HRMA of human clinical samples.

http://www.ijmm.org/text.asp?2018/36/1/65/231668

Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Helen Hencida Thangamony Ravindran Kumar Chinnappan Palaniappan Thangavelu Mani Mariappa Berlin Grace Viswanathan Mariammal Kootallur Narayanan Brahmadathan Source Type: research