Detection of retromer assembly in Plasmodium falciparum by immunosensing coupled to Surface Plasmon Resonance

Publication date: Available online 11 April 2018 Source:Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics Author(s): Mohd Shameel Iqbal, Asim Azhar Siddiqui, Chinmoy Banerjee, Shiladitya Nag, Somnath Mazumder, Rudranil De, Shubhra Jyoti Saha, Suresh Kumar Karri, Uday Bandyopadhyay Retromer complex plays a crucial role in intracellular protein trafficking and is conserved throughout the eukaryotes including malaria parasite, Plasmodium falciparum, where it is partially conserved. The assembly of retromer complex in RBC stages of malarial parasite is extremely difficult to explore because of its complicated physiology, small size, and intra-erythrocytic location. Nonetheless, understanding of retromer assembly may pave new ways for the development of novel antimalarials targeting parasite-specific protein trafficking pathways. Here, we investigated the assembly of retromer complex in P. falciparum, by an immunosensing method through highly sensitive Surface Plasmon Resonance (SPR) technique. After taking leads from the bioinformatics search and literature, different interacting proteins were identified and specific antibodies were raised against them. The sensor chip was prepared by covalently linking antibody specific to one component and the whole cell lysate was passed through it in order to trap the interacting complex. Antibodies raised against other interacting components were used to detect them in the trapped complex on the SPR chip. We were able ...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - Category: Biochemistry Source Type: research