High-throughput single-cell fate potential assay of murine hematopoietic progenitors in vitro

Recent single-cell transcriptomic studies have led to the proposal of new models for the hematopoietic hierarchy [1 –10]. Testing these models requires matching cell transcriptional state with functional cell fate potential at the single-cell level. Older studies have reported “low-throughput” single-cell differentiation assays in vitro, where outcome is measured using colony formation and morphological cri teria [11–13]. Recently, tracking of single hematopoietic stem cell differentiation by in vitro imaging has also been described [14,15], and index sorting was used to link single-cell transcriptomics with single-cell fate potential assays including single-cell transplantation [16,17].

Source: Experimental Hematology - February 3, 2018 Category: Hematology Authors: Betsabeh Khoramian Tusi, Merav Socolovsky Tags: Methods and Techniques Source Type: research