Iron released from reactive microglia by noggin improves myelin repair in the ischemic brain.

Iron released from reactive microglia by noggin improves myelin repair in the ischemic brain.
Neuropharmacology. 2018 Jan 30;:
Authors: Shin JA, Kim YA, Kim HW, Kim HS, Lee KE, Kang JL, Park EM
Abstract
We previously reported that the bone morphogenetic protein (BMP) antagonist, noggin, improved the repair process with an increase in the reactive microglia/macrophage population in the ischemic brain. Since BMP plays a role in intracellular iron homeostasis via the hepcidin/ferroportin axis, and iron is required for myelination, this study was aimed to determine whether noggin affected iron status and remyelination in the brain following ischemic stroke. We further examined the effect of blocking the BMP/hepcidin pathway on reactive microglia (BV2) and myelination of oligodendroglial cells (MO3.13) to define the link between microglial iron status and myelin formation. Following the noggin infusion into the ischemic brain of mice, the induction of hepcidin and ferritin protein levels decreased, and the number of myelinated axons and myelin thickness increased at 8 weeks after ischemic stroke. Noggin repressed the increase in hepcidin and ferritin levels in BV2 exposed to lipopolysaccharide (LPS) and oxygen/glucose deprivation and reperfusion (OGD/R). When MO3.13 were exposed to the conditioned media from noggin-treated BV2 (noggin CM) during reperfusion, OGD/R-induced MO3.13 cell death was reduced. Under normal conditions, noggin CM...

Source: Neuropharmacology - January 30, 2018 Category: Drugs & Pharmacology Authors: Shin JA, Kim YA, Kim HW, Kim HS, Lee KE, Kang JL, Park EM Tags: Neuropharmacology Source Type: research