GSE98192 Transcription of bovine milk and meat factors (BMMFs) in HEK293TT cells

Contributors : Sebastian Eilebrecht ; Agnes Hotz-Wagenblatt ; Victor Sarachaga ; Corinna Whitley ; Timo BundSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiens ; Sphinx1.76-related DNAWe analyze the transcription of these bovine milk and meat factors (BMMFs) and MS isolates in human HEK293TT cells. While all analyzed DNA agents are readily transcribed, one of the MS brain isolates (MSBI1.176), sharing homology with a transmissible spongiform encephalopathy (TSE)-associated DNA molecule, is transcribed at highest levels. Our results support the transcription of an open reading frame (ORF) encoding a replication-associated protein, which is highly conserved among all isolated BMMFs. In the case of the cow milk isolate CMI1.252, transcription also occurs in a second large ORF. Transcriptome profiling upon BMMF expression identified host cellular gene expression changes related to cell cycle progression and cell viability control, indicating potential pathways for a pathogenic involvement of BMMFs.

Source: GEO: Gene Expression Omnibus - February 5, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Sphinx1.76-related DNA Source Type: research