GSE84000 Specific metabolic activation of adipose tissue macrophages during obesity promotes inflammatory responses

Contributors : Lily Boutens ; Guido J Hooiveld ; Rinke StienstraSeries Type : Expression profiling by arrayOrganism : Mus musculusRecent studies have identified intracellular metabolism as a fundamental determinant of macrophage function. In obesity, proinflammatory macrophages accumulate in adipose tissue and trigger chronic low-grade inflammation, that promotes the development of systemic insulin resistance, yet changes in their intracellular energy metabolism are currently unknown. We therefore set out to study metabolic signatures of adipose tissue macrophages (ATMs) in lean and obese conditions. F4/80-positive ATMs were isolated from obese vs lean mice. High-fat feeding of wild-type mice and myeloid-specific Hif1 α-/- mice was used to examine the role of hypoxia-inducible factor-1α (HIF-1α) in ATMs part of obese adipose tissue. In vitro, bone marrow-derived macrophages were co-cultured with adipose tissue explants to examine adipose tissue-induced changes in macrophage phenotypes. Transcriptome analysis, real-time flux measurements, ELISA and several other approaches were used to determine the metabolic signatures and inflammatory status of macrophages. In addition, various metabolic routes were inhibited to determine their relevance for cytokine production. Transcriptome analysis and extracellular flux measurements of mouse ATMs revealed unique metabolic rewiring in obesity characterised by both increased glycolysis and oxidative phosphorylation. Simil...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research

Related Links:

Authors: Gougelet A Abstract Hepatocellular carcinoma (HCC) is the fifth most common cancer and the second cause of cancer-related death worldwide. The incidence of HCC is constantly increasing in correlation with the rise in diabetes and obesity, arguing for an urgent need for new developments in the treatment of this lethal cancer. Exosomes are small double-membrane vesicles loaded with distinct cargos, particularly small non-coding RNAs called microRNAs, representative of each donor cell and secreted to affect the features of neighboring cells or recipient cells located further away, like in the case of metastas...
Source: World Journal of Hepatology - Category: Gastroenterology Tags: World J Hepatol Source Type: research
We read with interest the recent paper by Wei, Jih-Hua et al.1 Briefly, authors evaluated cardiovascular disease risk after metabolic surgery in 392 type 2 diabetes mellitus patients using UKPDS score and investigated the efficacy of different bariatric/metabolic procedures on cardiovascular disease risk reduction.
Source: Surgery for Obesity and Related Diseases - Category: Surgery Authors: Source Type: research
CONCLUSIONS: These suggested the beneficial effects of vitamin D supplementation in obesity-related diabetes rat, which may through VDR, IRS-1/p-IRS-1, and GluT4 signaling activation. PMID: 30531693 [PubMed - as supplied by publisher]
Source: Minerva Endocrinologica - Category: Endocrinology Tags: Minerva Endocrinol Source Type: research
Polycystic ovary syndrome (PCOS) is a complex endocrine condition that represents a lifelong health concern for 1 in 10 women worldwide.1 PCOS is commonly diagnosed by the presence of ovulatory dysfunction, androgen excess, and/or ovaries with polycystic morphology on ultrasound examination.2 Half of patients experience overweight or obesity.3 Most are at increased risk for serious comorbidities, including infertility, pregnancy complications (eg, gestational diabetes mellitus), impaired glucose tolerance, insulin resistance, type 2 diabetes mellitus, cardiovascular disease, gynecological cancers, anxiety, depression, eati...
Source: Journal of the American Dietetic Association - Category: Nutrition Authors: Tags: Research Source Type: research
Conclusion: There are differences according to gender, and, in general, women accumulate more protective factors and men, more risk factors.
Source: Revista Brasileira de Epidemiologia - Category: Epidemiology Source Type: research
Resumo Este trabalho objetiva avaliar os marcadores do consumo alimentar de pacientes diab éticos por meio de um aplicativo móvel. Estudo transversal, realizado com 100 adultos e idosos portadores de Diabetes Mellitus tipo 2, internados em um hospital do interior do estado do Rio Grande do Sul. Para a coleta de dados utilizou-se o aplicativo móvel Diabetes FoodControl. Na associação da adequação dos marcadores do consumo com as variáveis investigadas, utilizou-se o teste do qui-quadrado ao nível 5% de significância. Identificou-se alto percentual de indiv...
Source: Ciencia e Saude Coletiva - Category: Occupational Health Source Type: research
CONCLUSIONS Half of the population studied had adequate control of hypertension. The improvement of this control is an important challenge, which should consider overcoming social and regional inequalities associated with it.RESUMO OBJETIVO Estimar a preval ência do controle adequado da hipertensão arterial sistêmica entre adultos mais velhos e examinar a sua associação com fatores predisponentes, facilitadores e de necessidade para o uso de serviços de saúde. MÉTODOS A análise foi conduzida em 4.148 participantes ( ≥ 50 anos) da linha de base do Estudo Longit...
Source: Revista de Saude Publica - Category: International Medicine & Public Health Source Type: research
Publication date: January–February 2019Source: Diabetes &Metabolic Syndrome: Clinical Research &Reviews, Volume 13, Issue 1Author(s): Mohammad Sadaat Bukht, Kazi Rumana Ahmed, Sahadat Hossain, Parisha Masud, Shuhana Sultana, Rasheda KhanamAbstractBackgroundThe aim of the study was to assess the association of physical activity (PA) with the presence of diabetes related complications among Bangladeshi type 2 diabetic subjects.MethodsThis cross-sectional study was conducted in the Out Patient Department (OPD) of BIRDEM, Bangladesh. A group of 977 subjects were randomly selected and followed up. Diabetes was dia...
Source: Diabetes and Metabolic Syndrome: Clinical Research and Reviews - Category: Endocrinology Source Type: research
This study examined the risk factors for type 2 diabetes in multiethnic urban youth in Edmonton, Alberta.MethodsAn interviewer-administered questionnaire was developed based on CANRISK variables, such as age, gender, ethnicity, family history of diabetes, medical history of high blood sugar or high blood pressure, anthropometric measurements, physical activity and dietary intake. Between October 2013 and March 2014, data were collected from a convenience sample of 557 (328 girls and 229 boys) multiethnic youth 11 to 23 years of age in 12 institutions in Edmonton, such as public schools, after-school programs and colle...
Source: Canadian Journal of Diabetes - Category: Endocrinology Source Type: research
Abstract BACKGROUND: Professional drivers are a group exposed to many cardiovascular risk factors. Non-systematic working hours, prolonged stress, low physical activity, along with irregular, and in most cases, unhealthy meals are common aspects of the normal working schedule of most of the professional drivers. These translate into high risk of cardiovascular disease (CVD). AIM: The aim of the current analysis was to establish the prevalence of dyslipidaemia in a group of continuous professional drivers. METHODS: The RACER (Risk of Adverse Cardiovascular Events among professional dRivers in Poland - Amb...
Source: Kardiologia Polska - Category: Cardiology Authors: Tags: Kardiol Pol Source Type: research
More News: Diabetes | Eating Disorders & Weight Management | Endocrinology | Genetics | High Fat | Insulin | Obesity | Study