Evaluation of a pan-Leishmania Spliced-Leader RNA Detection Method in Human Blood and Experimentally-Infected Syrian Golden Hamsters

This study compared the limits of detection of various real-time PCR assays in hamsters infected with Leishmania infantum, in spiked human blood and in clinical blood samples from visceral leishmaniasis patients. The SL-RNA assay showed an excellent analytical sensitivity in tissues (0.005 and 0.002 parasites per mg liver and spleen, respectively) and was not prone to false-positive reactions.

Source: Journal of Molecular Diagnostics - January 17, 2018 Category: Pathology Authors: Eline Eberhardt, Magali Van den Kerkhof, Dimitri Bult é, Dorien Mabille, Lieselotte Van Bockstal, Séverine Monnerat, Fabiana Alves, Jane Mbui, Peter Delputte, Paul Cos, Sarah Hendrickx, Louis Maes, Guy Caljon Tags: Regular article Source Type: research