Revisiting the thiosemicarbazonecopper(II) reaction with glutathione. Activity against colorectal carcinoma cell lines.

Revisiting the thiosemicarbazonecopper(II) reaction with glutathione. Activity against colorectal carcinoma cell lines.
J Inorg Biochem. 2017 Dec 08;180:69-79
Authors: García-Tojal J, Gil-García R, Fouz VI, Madariaga G, Lezama L, Galletero MS, Borrás J, Nollmann FI, García-Girón C, Alcaraz R, Cavia-Saiz M, Muñiz P, Palacios Ò, Samper KG, Rojo T
Abstract
Thiosemicarbazones (TSCs), and their copper derivatives, have been extensively studied mainly due to the potential applications as antitumor compounds. A part of the biological activity of the TSC-CuII complexes rests on their reactivity against cell reductants, as glutathione (GSH). The present paper describes the structure of the [Cu(PTSC)(ONO2)]n compound (1) (HPTSC=pyridine-2-carbaldehyde thiosemicarbazone) and its spectroscopic and magnetic properties. ESI studies performed on the reaction of GSH with 1 and the analogous [{Cu(PTSC*)(ONO2)}2] derivative (2, HPTSC*=pyridine-2-carbaldehyde 4N-methylthiosemicarbazone) show the absence of peaks related with TSC-Cu-GSH species. However GSH-Cu ones are detected, in good agreement with the release of CuI ions after reduction in the experimental conditions. The reactivity of 1 and 2 with cytochrome c and myoglobin and their activities against HT-29 and SW-480 colon carcinoma cell lines are compared with those shown by the free HPTSC and HPTSC* ligands.
PMID: 29247869 [PubMed - as supplied by publisher]

Source: Journal of Inorganic Biochemistry - December 8, 2017 Category: Biochemistry Authors: García-Tojal J, Gil-García R, Fouz VI, Madariaga G, Lezama L, Galletero MS, Borrás J, Nollmann FI, García-Girón C, Alcaraz R, Cavia-Saiz M, Muñiz P, Palacios Ò, Samper KG, Rojo T Tags: J Inorg Biochem Source Type: research