Harnessing the periplasm of bacteria to develop biocatalyst for biosynthesis of highly pure chemicals.

Harnessing the periplasm of bacteria to develop biocatalyst for biosynthesis of highly pure chemicals. Appl Environ Microbiol. 2017 Oct 27;: Authors: Yang Y, Wu Y, Hu Y, Wang H, Guo L, Fredrickson JK, Cao B Abstract Although biocatalytic transformation has shown great promise in chemical synthesis, there remain significant challenges in controlling high selectivity without the formation of undesirable byproducts. For instance, few attempts have been successful in constructing biocatalyst for de novo synthesis of pure flavin mononucleotide (FMN) due to riboflavin (RF) accumulating inside the cytoplasm and being secreted with FMN. To address this problem, we show here a novel biosynthesis strategy, compartmentalizing the final FMN biosynthesis step in the periplasm of an engineered Escherichia coli strain. This construct is able to over-produce FMN with high specificity (92.4% of total excreted flavins). Such biosynthesis approach allows isolation of the final biosynthesis step from the cytoplasm so as to eliminate undesirable by-products, providing a new route to develop biocatalysts for high-purity chemicals.IMPORTANCE: The periplasm of Gram-negative bacterial hosts is engineered to compartmentalize the final biosynthesis step from the cytoplasm. This strategy is promising for the overproduction of high-value product with high specificity. We demonstrate the successful implementation of this strategy in microbial production of highly...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research