Activation of hypoxia-inducible factor-1 in rat brain after perinatal anoxia: role of body temperature.

Activation of hypoxia-inducible factor-1 in rat brain after perinatal anoxia: role of body temperature. Int J Hyperthermia. 2017 Oct 04;:1-26 Authors: Kletkiewicz H, Hyjek M, Jaworski K, Nowakowska A, Rogalska J Abstract Transcriptional hypoxia-inducible factor-1α (HIF-1α) plays the fundamental role in adaptive processes in response to hypoxia. Specific HIF-1α target genes are involved in glycolysis, erythropoiesis and angiogenesis to promote survival. In our previous study we have demonstrated that naturally low body temperature of newborn rats protects them against damage due to perinatal hypoxia. Therefore, our experiments aimed at checking the effects of body temperature during simulated perinatal anoxia on subsequent changes of expression of HIF-1α and its specific target genes such as VEGF and EPO in the rat brain. Two-day old Wistar rats were divided into 3 temperature groups: normothermic -33°C, hyperthermic - 37°C and extremely hyperthermic - 39°C. The temperature was controlled starting 15 min before and continuing during 10 min of anoxia as well as for 2 h post-anoxia. HIF-1α was analyzed by Western blot and immunofluorescence and mRNA levels of HIF-1α and its downstream genes (VEGF, EPO) were quantified by qRT-PCR. Thermal conditions during neonatal anoxia affected the hippocampal and neocortical level of HIF-1α protein. Physiological body temperature of newborn rats led to prominent accumulation of cerebr...
Source: International Journal of Hyperthermia - Category: Internal Medicine Tags: Int J Hyperthermia Source Type: research