Abstract 143: Pregnancy Dependant Transcriptomic Changes in Uterine Arteries From Hypertensive and Normotensive Rat Models [Session Title: Developmental Programming and Pregnancy Induced Hypertension]

This study investigated early gene expression changes in the uterine arteries (UA) of pregnant SHRSP and normotensive Wistar Kyoto (WKY) rats.Methods: SHRSP and WKY females were time mated and UA isolated at gestational day (GD)6 (n=3). Non-pregnant (NP) UA were isolated from virgin aged matched controls (n=3). UA RNAseq (Illumina platform) was performed. Transcript level gene changes were interpreted using Ingenuity Pathway Analysis (FDR 1.0). Quantitative RT-PCR was used to validate significantly differentially expressed genes.Results: There was a greater number of differentially expressed transcripts NP v GD6 in SHRSP compared to WKY (796 vs 535). 147 pregnancy dependant gene changes were common to SHRSP and WKY; which involved cellular movement and cell growth/proliferation pathways. Further analysis revealed calcium signalling genes involved in the α-adrenergic pathway were downregulated in WKY NP v GD6, e.g. inositol-1,4,5-trisphosphate receptor (Ip3r) (FC 2.5; p
Source: Hypertension - Category: Cardiology Authors: Tags: Oral Abstract Presentations Source Type: research