Quantitating native hematopoiesis

Recently, experimental tools have been developed for non-invasive labeling of hematopoietic stem cells (HSC), allowing the study of hematopoiesis at high resolution without experimental perturbation. This talk is based on data from mouse models developed in the Rodewald laboratory for in vivo fate mapping of HSC (using inducible Cre expressed from the Tie2 locus) and for endogenous, Cre-driven barcoding (using Polylox, a novel loxP-based barcode generator). I will address the following questions: (1) What are the cellular sources of definitive hematopoiesis in development and its maintenance during adulthood and aging, and how active are these sources? (2) What is the topology of differentiation pathways emerging from HSC in development and adulthood? (3) How is the size of the stem cell pool maintained – by asymmetric division of single HSC or at the level of the HSC population? The in vivo fate-mapping and barcoding data lend themselves to quantitative analyses and data-driven mathematical modeling that shed light on these questions.

Source: Experimental Hematology - August 22, 2017 Category: Hematology Authors: Thomas H öfer, Melania Barile, Katrin Busch, Thorsten Feyerabend, Weike Pei, Jens Rössler, Ann-Kathrin Schuon, Xi Wang, Hans-Reimer Rodewald Source Type: research