Modeling human hematopoiesis in pluripotent stem cells

Hematopoietic stem cell (HSC) transplantation reconstitutes the blood cell compartment following myeloablative therapy or for patients with marrow aplasia. Because many patients do not have an optimal matched donor, the provision of HSCs from alternate sources, such as differentiated human pluripotent stem cells (hPSCs), is required. Despite considerable efforts, it has not been possible to efficiently generate repopulating HSCs from PSCs. Comparing the transcriptional profiles of hPSC-derived hematopoietic progenitors and repopulation-competent cord blood progenitors, we determined that failure to express HOXA genes in the hPSC progenitors was a major difference between the two populations.

Source: Experimental Hematology - August 22, 2017 Category: Hematology Authors: Andrew Elefanty, Elizabeth Ng, Freya Bruveris, Lisa Azzola, Belinda Phipson, Katerina Vlahos, Ana Rita Leuitoguinho, Vincenzo Calvanese, Katja Schenke-Layland, Alicia Oshlack, Hanna Mikkola, Edouard Stanley Source Type: research