Structural integrity and viability of Fredericella sultana statoblasts infected with Tetracapsuloides bryosalmonae (Myxozoa) under diverse treatment conditions

AbstractFredericella sultana is an invertebrate host ofTetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids. The bryozoan produces seed-like statoblasts to facilitate its persistence during unfavourable conditions. Statoblasts from infected bryozoans can harborT. bryosalmonae and give rise to infected bryozoan colonies when conditions improve. We aimed in the present study to evaluate the integrity and viability ofT. bryosalmonae-infected statoblasts after a range of harsh treatment conditions. We tested if statoblasts could survive ingestion by either brown trout or common carp. After ingestion, the fish faeces was collected at different time points. We also tested physical stressors: statoblasts collected from infected colonies were desiccated at room temperature, or frozen with and without Bryozoan Medium C (BMC). After treatments, statoblasts were assessed for physical integrity before being incubated on BMC to allow them to hatch. After 4  weeks, hatched and unhatched statoblasts were tested by PCR for the presence of the parasite. We found that statoblasts ingested by brown trout and those frozen in BMC were completely broken. In contrast, statoblasts ingested by common carp and those subjected to dry freezing were able to survive and hatch.T. bryosalmonae was detected by PCR in both hatched and unhatched infected statoblasts, but neither from broken nor uninfected statoblasts. Our results confirmed for the first time the ...
Source: Veterinary Research - Category: Veterinary Research Source Type: research

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[Epidemiology of Cutaneous Leishmaniasis in Algeria through Molecular Characterization]. Bull Soc Pathol Exot. 2019;112(3):147-152 Authors: Bachi F, Icheboudene K, Benzitouni A, Taharboucht Z, Zemmouri M Abstract Three distinct noso-epidemiological cutaneous leishmaniasis (LC) entities coexist in Algeria: the so-called sporadic form of the North (LCN), the zoonotic form (LCZ) and the chronic form (LCC). The precise identification of the parasitic species involved in each of the forms makes it possible to specify the geographical distribution of each of the forms raised, to distinguish their c...
Source: Bulletin de la Societe de Pathologie Exotique - Category: Tropical Medicine Tags: Bull Soc Pathol Exot Source Type: research
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Source: Emerging Infectious Diseases Journal - Category: Infectious Diseases Source Type: research
(Lancaster University) Researchers have shed light on how the parasite which causes sleeping sickness multiples inside its host. Human African Trypanosomiasis or sleeping sickness, only occurs in Sub-Saharan Africa where an estimated 60 million people in 36 countries are at risk. The infection attacks the central nervous system and is fatal without treatment. Lancaster University researchers have found that the parasite's cell division differs from that of humans and animals.
Source: EurekAlert! - Infectious and Emerging Diseases - Category: Infectious Diseases Source Type: news
Development of effective malaria vaccines requires delivery platforms to enhance the immunogenicity and efficacy of the target antigens. This is particularly challenging for transmission-blocking malaria vaccines (TBVs), and specifically for those based on the Pfs25 antigen, that need to elicit very high antibody titers to stop the parasite development in the mosquito host and its transmission. Presenting antigens to the immune system on virus-like particles (VLPs) is an efficient way to improve the quantity and quality of the immune response generated. Here we introduce for the first time a new VLP vaccine platform, based...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
ConclusionsThe authors are uncertain whether or not nitazoxanide could improve the excretion rate of pathogens. Based on low-certainty evidence, nitazoxanide may improve the remission rate of diarrhea in children with intestinal parasite infections, but it may be associated with an increased risk of adverse reactions. Hence, more RCTs with a low risk of bias are still needed to assess the efficacy and safety of nitazoxanide.
Source: Indian Journal of Pediatrics - Category: Pediatrics Source Type: research
ve;ndez-Busquets Gomes Recently, we disclosed primaquine cell penetrating peptide conjugates that were more potent than parent primaquine against liver stage Plasmodium parasites and non-toxic to hepatocytes. The same strategy was now applied to the blood-stage antimalarial chloroquine, using a wide set of peptides, including TP10, a cell penetrating peptide with intrinsic antiplasmodial activity. Chloroquine-TP10 conjugates displaying higher antiplasmodial activity than the parent TP10 peptide were identified, at the cost of an increased hemolytic activity, which was further confirmed for their primaquine analogues...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
by Malgorzata A. Domagalska, Hideo Imamura, Mandy Sanders, Frederik Van den Broeck, Narayan Raj Bhattarai, Manu Vanaerschot, Ilse Maes, Erika D ’Haenens, Keshav Rai, Suman Rijal, Matthew Berriman, James A. Cotton, Jean-Claude Dujardin Whole genome sequencing (WGS) is increasingly used for molecular diagnosis and epidemiology of infectious diseases. CurrentLeishmania genomic studies rely on DNA extracted from cultured parasites, which might introduce sampling and biological biases into the subsequent analyses. Up to now, direct analysis ofLeishmania genome in clinical samples is hampered by high levels of human DNA a...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
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Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
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Source: Parasitology International - Category: Parasitology Source Type: research
Publication date: Available online 11 December 2019Source: Journal of Molecular StructureAuthor(s): Terence J. Noonan, Kelly Chibale, Susan A. Bourne, Mino R. CairaAbstractA crystal engineering approach combined with experimental screening methods was employed as a preformulation step for producing expectedly useful forms of a novel antimalarial drug lead (MMV652103). In previous experiments, this drug lead had shown promise both in vitro and in vivo against the human malaria parasite Plasmodium falciparum. Various new forms of MMV652103 were produced earlier, including co-crystals, a salt, polymorphs and a hydrated form a...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
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