A QCM-D and SAXS Study of the Interaction of Functionalised Lyotropic Liquid Crystalline Lipid Nanoparticles with siRNA.

A QCM-D and SAXS Study of the Interaction of Functionalised Lyotropic Liquid Crystalline Lipid Nanoparticles with siRNA. Chembiochem. 2017 Feb 23;: Authors: Tajik-Ahmadabad B, Mechler A, Muir BW, McLean K, Hinton TM, Separovic F, Polyzos A Abstract Biophysical studies were undertaken to investigate the binding and release of short interfering ribonucleic acid (siRNA) from lyotropic liquid crystalline lipid nanoparticles (LNPs) using a quartz crystal microbalance (QCM). These carriers are based on phytantriol (Phy) and a cationic lipid, DOTAP (1, 2-dioleoyl-3 trimethylammonium propane). The non-lamellar phase LNPs were tethered to the surface of the QCM chip for analysis based on biotin-neutravidin binding, which enabled the controlled deposition of siRNA-LNP complexes with different lipid/siRNA charge ratios on a QCM-D crystal sensor. The binding and release of biomolecules such as siRNA from LNPs was demonstrated to be reliably characterized using this technique. Essential physicochemical parameters of the cationic LNP/siRNA lipoplexes, such as particle size, lyotropic mesophase behavior, cytotoxicity, gene silencing and uptake efficiency, were also assessed. The SAXS data show that upon lowering the pH to 5.5, the structure of lipoplexes did not change, indicating that the acidic conditions of the endosome were not a significant factor in the release of siRNA from the cationic lipidic carriers. PMID: 28233412 [PubMed - as s...
Source: Chembiochem - Category: Biochemistry Authors: Tags: Chembiochem Source Type: research