CagA-positive and CagA-negative Helicobacter pylori strains differentially affect the expression of micro RNAs 21, 92a, 155 and 663 in human umbilical vein endothelial cells.

CagA-positive and CagA-negative Helicobacter pylori strains differentially affect the expression of micro RNAs 21, 92a, 155 and 663 in human umbilical vein endothelial cells. Cell Mol Biol (Noisy-le-grand). 2017 Jan 30;63(1):34-40 Authors: Kalani M, Hodjati H, GhamarTalepoor A, Samsami Dehaghani A, Doroudchi M Abstract Given that the basic mechanism of the effect of Helicobacter (H.) pylori in the induction of atherosclerosis remains unknown and regarding the regulatory role of micro RNAs (miRNAs) in endothelial cell (EC) functions, we aimed to investigate the effect of H. pylori on the expression of miRNAs involved in atherosclerosis (atheromiRs) and their correlation with apoptosis in human umbilical vein EC (HUVEC). HUVECs were treated with different cytotoxin associated gene A (CagA) positive and negative H. pylori derived products, then the levels of apoptosis and miR-21, 92a, 155 and 663 were measured using flowcytometry and real time-PCR methods, respectively. Although, comparing induced apoptosis and necrosis in HUVECs revealed that water extract of CagA+ H. pylori (HpWE) was more potent than CagA- one and H. pylori lipopolysacharide (Hp-LPS), no significant difference was observed between LPS extracted from CagA+ and CagA- strains. Besides, CagA+ HpWE significantly increased the levels of anti-apoptotic miR-21, and inflammatory miRNAs 155 and 663 but not miR-92a. A positive correlation was observed between apoptosis and necr...
Source: Cellular and Molecular Biology - Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research