Multifunctional superparamagnetic nanoparticles: from synthesis to siRNA delivery.

Multifunctional superparamagnetic nanoparticles: from synthesis to siRNA delivery. Curr Pharm Des. 2016 Oct 31; Authors: Arami S, Mahdavi M, Rashidi MR, Fathi M, Hejazi MS, Samadi N Abstract Targeted delivery of small interfering RNA (siRNA) to specific tumor tissues and cells remains as one of the key challenges in the development of RNA interference as a therapeutic application. To target breast cancer, we developed a cationic nanoparticle as a therapeutic delivery system. The successful synthesis of the magnetic nanoparticles modified by polyaspartate (PAA) and polyethyleneimine (PEI) was confirmed using fourier transform infrared (FT-IR) measurements. The designed nanoparticle has been characterized evaluating its size and charge before and after nanoplex formation with siRNA. The designed nanoparticle could effectively form nanoplex with siRNA in 2:1 w/w ratio. Survivin siRNA was used to suppress the antiapoptotic gene, survivin, in MCF-7 cells. According to the importance of combinational therapy, Mitoxantrone (MTX) was used as a chemotherapeutic agent beside. The multifunctional nanoparticles have been successfully entered into about 63% of the MCF-7 cells which was shown via microscopic and flowcytometric methods. Effective cellular uptake led the cells to apoptosis. Down regulation of survivin was determined in mRNA and protein levels via Real Time PCR and western blotting. Gathering all obtained data, we came to conclusion ...
Source: Current Pharmaceutical Design - Category: Drugs & Pharmacology Authors: Tags: Curr Pharm Des Source Type: research