Serogenotyping and emergence of extended spectrum beta lactamase genes in non-typhoidal Salmonella: first report from Saudi Arabia.

This study describes the molecular characterization of NTS in Saudi Arabia by serogenotyping and identification of ESBL genes. Forty-eight multidrug resistant NTS identified at a tertiary care facility in Saudi Arabia from 2007-2014 were studied. Bacterial identification, antibiotic resistance and classical O-antigen serogrouping were performed using standard methods. Serogenotyping was carried out using the Salm-SeroGenoTyping AS-1 Kit (Alere Technologies GmbH, Jena, Germany). Characterization of ESBL and carbapenemase genes was carried out using the Check-MDR CT103 DNA microarray (CheckPoints BV, Wageningen, Netherlands). With the exception of one S. enterica subsp. salamae, all isolates were S. enterica subsp. enterica for which 19 serovars were identified by serogenotyping. Predominant serovars were: Salmonella Enteritidis (n=16), Salmonella Typhimurium (n=9) and Salmonella Infantis (n=4). Genes associated with beta lactam resistance detected were blaTEM1, blaPSE-1, blaCTX-M, blaOXA-1 and blaCMY. The blaCTX-M ESBL identified were CTX-M1, CTX-M2; CTX-M8; CTX-M9; CTX-M15; CTX-M26. Majority of isolates (n=10) harboured one ESBL gene. One isolate harboured four blaCTX-M genes (CTX-M2, -M8, -M9, -M26). No isolate harboured carbapenemase genes. This is the first report of ESBL genes among Salmonella isolates in Saudi Arabia and the identification of blaCTX-M2, blaCTX-M8 and blaCTX-M26 in Salmonella isolates in the Arabian Gulf region. PMID: 27655242 [PubMed - as supplie...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research