Temporal deletion of Aqp11 in mice is linked to the severity of cyst-like disease.

This study examined 1) whether postnatal deletion of Aqp11 affects PT injury and cyst formation, 2) the temporal role of Aqp11 deletion on cyst development, and 3) the nature of apparent cysts. Tamoxifen-inducible Aqp11(-/-) mice were generated (Ti-Aqp11(-/-)). Deletion of Aqp11 at post-natal (P) days 2, 4, 6, 8 and 12 was investigated. Deranged renal development, especially in kidney cortex, PT cell vacuolization and apparent tubular cysts developed only in mice where Aqp11 gene disruption was induced until P8. Aqp11 gene deletion from P12 onwards did not result in a clear deficiency in renal development, PT injury or cyst formation. Intra-peritoneal injection of biotinylated-dextran (10 kDa) into adult mice resulted in extensive endocytic dextran uptake in both cystic Aqp11(-/-) and control PT epithelium, respectively. This suggests that apparent cysts are not membrane enclosed structures but represent PT dilations. We conclude that Aqp11(-/-) mice develop cyst-like dilated proximal tubules without documented cysts at time of death. PMID: 27582095 [PubMed - as supplied by publisher]
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research