Distribution of PCR-ribotypes among recent Clostridium difficile isolates collected in two districts of Hungary using capillary gel electrophoresis and review of changes in the circulating ribotypes over time.

Distribution of PCR-ribotypes among recent Clostridium difficile isolates collected in two districts of Hungary using capillary gel electrophoresis and review of changes in the circulating ribotypes over time. J Med Microbiol. 2016 Aug 17; Authors: Tóth J, Urbán E, Osztie H, Benczik M, Indra A, Nagy E, Allerberger F Abstract Following the first description of a Clostridium difficile case caused by ribotype 027 in Hungary in 2007, the rapid spread of C. difficile infection in different hospitals within the country was observed. The aim of this pilot study was to investigate the distribution of different PCR-ribotypes among inpatient and outpatient isolates obtained in two geographically different parts of Hungary. One hundred and ninety-two toxigenic C. difficile isolates collected between 1st October and 1st December, 2014 were PCR-ribotyped using capillary gel electrophoresis and the database of WEBRIBO (http://webribo.ages.at) which allows the automatic analysis and comparison of capillary-sequencer-based PCR-ribotyping-data. Altogether 31 different known ribotypes were found and 16 isolates showed a novel banding pattern, not included in the current library. Beside the dominance of 027 (33.3 %) among all isolates there were differences in its presence among isolates obtained from the two regions (45.8 % in the Central region, and 20.8 % in the South-east region, respectively). Whereas the second most prevalent ribotype 036 (19.8...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research