Xenograft of Mus musculus ovary in the uterus of Rattus norvegicus after induction of pseudopregnancy

AbstractXenograft of gonads such as ovarian tissue can preserve fertility in patients at risk of infertility after radiotherapy or chemotherapy and also is valuable in preserving fertility in mammalian species. The purpose of this investigation was to preserve mouse (Mus musculus) ovarian tissue after xenografting in uterine lumen of rat (Rattus norvegicus) immunoprivileged by pseudopregnancy. Ten adult female Balb/c mice and seven female and four male Sprague-Dawley rats were enrolled. Seven mice were selected for transplantation, and three mice were euthanized and their ovaries were removed as controls. Female Sprague-Dawley rats in proestrous stage were mated with vasectomized male rats to induce pseudopregnancy 7  days after mating. Both ovaries of mice in diestrous stage were simultaneously and gently transferred into the uterine lumen of pseudopregnant rat. Fourteen days after xenotransplantation, the ovaries were removed and were histologically evaluated. Fourteen days after xenotransplantation, four out of seven transplanted mice ovaries were not rejected in rat uterine cavities. From 14 ovaries, eight ovaries could survive in the uterine lumen and were attached to the endometrial layer of rat uterus. Six ovaries lacked active large follicles and corpora lutea but no evidence of tissue necrosis wa s noted. Two ovaries showed evidence of inactive corpora lutea, secondary follicles. Also, primordial follicles were detectable in these two ovaries. Capillaries filled wit...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research