Development and evaluation of a multi-locus sequence typing scheme for Mycoplasma synoviae.

Development and evaluation of a multi-locus sequence typing scheme for Mycoplasma synoviae. Avian Pathol. 2016 Feb 29;:1-37 Authors: Dijkman R, Feberwee A, Landman WJ Abstract Reproducible molecular Mycoplasma synoviae typing techniques with sufficient discriminatory power may help to expand knowledge on its epidemiology and contribute to the improvement of control and eradication programmes of this mycoplasma species. The present study describes the development and validation of a novel multi-locus sequence typing (MLST) scheme for M. synoviae. Thirteen M. synoviae isolates originating from different poultry categories, farms and lesions, were subjected to whole genome sequencing. Their sequences were compared to that of M. synoviae reference strain MS53. A high number of SNPs indicating considerable genetic diversity were identified. SNPs were present in over 40 putative target genes for MLST of which 5 target genes were selected (nanA, uvrA, lepA, ruvB and ugpA) for the MLST scheme. This scheme was evaluated analyzing 209 M. synoviae samples from different countries, categories of poultry, farms and lesions. Eleven clonal clusters and 76 different sequence types were obtained. Clustering occurred following geographical origin, supporting the hypothesis of regional population evolution. Further, M. synoviae samples obtained from epidemiologically linked outbreaks often harboured the same sequence type. In contrast, multiple lineage...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research