Combination of small RNAs for skeletal muscle regeneration [Research Communication]

In this study, we used myostatin small interfering (si)RNA (siGDF-8), a major inhibitory factor in the development and postnatal regeneration of skeletal muscle and muscle-specific microRNAs (miR-1 and -206) to further accelerate muscle regeneration. This combination of 3 small RNAs significantly improved the gene expression of myogenic regulatory factors in vitro, suggesting myogenic activation. Moreover, cell proliferation and myotube formation improved without compromising each other, which indicates the myogenic potential of this combination of small RNAs. The recovery of chemically injured tibialis anterior muscles in rats was significantly accelerated, both functionally and structurally. This novel combination of siRNA and miRNAs has promising therapeutic potential to improve in situ skeletal muscle regeneration.—Kim, N., Yoo, J. J., Atala, A., Lee, S. J. Combination of small RNAs for skeletal muscle regeneration.

Source: FASEB Journal - March 1, 2016 Category: Biology Authors: Kim, N., Yoo, J. J., Atala, A., Lee, S. J. Tags: Research Communication Source Type: research