Inhibition of C. albicans biofilm formation and modulation of gene expression by probiotic cells and supernatant.

In this study, combinations of Lactobacillus plantarum SD5870, L. helveticus CBS N116411, and Streptococcus salivarius DSM 14685 were assessed for their ability to inhibit the formation of, and disrupt C. albicans biofilms. Co-incubation with probiotic supernatants under hyphae-inducing conditions reduced C. albicans biofilm formation by over 75% in all treatment groups. Likewise, combinations of live probiotics reduced biofilm formation of C. albicans by over 67%. When live probiotics or their supernatants were overlaid on preformed C. albicans biofilms, biofilm size was reduced by over 63% and 65% respectively. Quantitative RT-PCR results indicated that the combined supernatants of SD5870 and CBS N116411 significantly reduced the expression of several C. albicans genes involved in the yeast-hyphae transition: the ALS3 adhesin/invasin by 70% (p<0.0001); the EFG1 hyphae-specific gene activator by 47% (p=0.0061); the SAP5 secreted protease by 49% (p<0.0001); and the HWP1 hyphal wall protein critical to biofilm formation by over 99% (p<0.0001). These findings suggest the combination of L. plantarum SD5870, L. helveticus CBS N116411, and S. salivarius DSM 14685 is effective at both preventing the formation of, and removing preformed C. albicans biofilms. Our novel results point to the downregulation of several Candida genes critical to the yeast-hyphae transition, biofilm formation, tissue invasion, and cellular damage. PMID: 26847045 [PubMed - as supplied by pu...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research