Membrane Trafficking of Collecting Duct Water Channel Protein AQP2 Regulated by Akt/AS160.

In this study, we demonstrated that 1) small interfering RNA (siRNA)-mediated gene silencing of Akt1 significantly decreased Akt1 and phospho-AS160 protein expression; and 2) confocal laser scanning microscopy of AQP2 in mouse cortical collecting duct cells (M-1 cells) revealed AS160 knockdown by siRNA increased AQP2 expression in the plasma membrane compared with controls, despite the absence of dDAVP stimulation. Thus, the results suggest that PI3K/Akt pathways could play a role in AQP2 trafficking via the AS160 protein. PMID: 21468198 [PubMed]
Source: Electrolyte and Blood Pressure - Category: Internal Medicine Tags: Electrolyte Blood Press Source Type: research