Development of a hybridoma cell line secreting monoclonal antibody against S protein of a Chinese variant of PEDV.
Development of a hybridoma cell line secreting monoclonal antibody against S protein of a Chinese variant of PEDV.
Monoclon Antib Immunodiagn Immunother. 2015 Feb;34(1):12-6
Authors: Lei X, Zhao J, Wang X, Zhao Y, Wang C
Abstract
Gene encoding 22-380 aa of spike protein of porcine epidemic diarrhea virus (PEDV) isolate in China was cloned and expressed as a recombinant protein in Escherichia coli BL21(DE3). Female BALB/c mice were immunized with the purified recombinant S protein, and a monoclonal antibody (MAb) designated as 2D1 against S protein was achieved by hybridoma technique. MAb 2D1 reacted with S protein of PEDV specifically. The monoclonal antibody 2D1 may provide a useful tool as a specific diagnostic reagent for detecting S protein of the Chinese variant of PEDV and for further investigation into the virus' pathogenic mechanism.
PMID: 25723278 [PubMed - indexed for MEDLINE]
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research