Anthelmintic efficacy of an organic fraction from Guazuma ulmifolia leaves and evaluation of reactive oxidative stress on Haemonchus contortus
This study describes the anthelmintic efficacy of an organic fraction (EtOAc- F) from Guazuma ulmifolia leaves and evaluation of its reactive oxidative stress on Haemonchus contortus. The first step was to assess the anthelmintic effect of EtOAc-F at 0.0, 3.5, 7.0 and 14 mg kg of body weight (BW) in gerbil's (Meriones unguiculatus) artificially infected with H. contortus infective larvae (L3). The second step was to evaluate the preliminary toxicity after oral administration of the EtOAc-F in gerbils. Finally, the third step was to determine the relative expression of biomarkers as glutathione (GPx), catalase (CAT), and superoxide dismutase (SOD) against H. contortus L3 post-exposition to EtOAc-F. Additionally, the less-polar compounds of EtOAc-F were identified by gas mass spectrophotometry (GC-MS). The highest anthelmintic efficacy (97.34%) of the organic fraction was found in the gerbils treated with the 14 mg/ kg of BW. Histopathological analysis did not reveal changes in tissues. The relative expression reflects overexpression of GPx (p˂0.05, fold change: 14.35) and over expression of SOD (p˂0.05, fold change: 0.18) in H. contortus L3 exposed to 97.44 mg/mL of EtOAc-F compared with negative control. The GC-MS analysis revealed the presence of 4-hydroxybenzaldehyde (1), leucoanthocyanidin derivative (2), coniferyl alcohol (3), ferulic acid methyl ester acetate (4), 2,3,4-trimethoxycinnamic acid (5) and epiyangambin (6) as major compounds. According to these results, the...
Source: Experimental Parasitology - Category: Parasitology Authors: Guillermo Res éndiz-González Agust ín Olmedo-Juárez Roberto Gonz ález-Garduño Jorge Alberto Cortes Morales Manas és González Cortazar Ana Elvia S ánchez-Mendoza Mar ía Eugenia López-Arellano Cris óforo Mercado-Márquez Alejandro Lara-Bueno Ros Source Type: research