Ssc-miR-7139-3p suppresses foot-and-mouth disease virus replication by promoting degradation of 3C < sup > pro < /sup > through targeting apoptosis-negative regulatory gene Bcl-2

Virology. 2024 Apr 16;595:110070. doi: 10.1016/j.virol.2024.110070. Online ahead of print.ABSTRACTFoot-and-mouth disease is a highly contagious and infectious disease affecting cloven-hoofed animals. Disease control is complicated by its highly contagious nature and antigenic diversity. Host microRNAs (miRNAs) are post-transcriptional regulators that either promote or repress viral replications in virus infection. In the present study, we found that ssc-miR-7139-3p (Sus scrofa miR-7139-3p) was significantly up-regulated in host cells during foot-and-mouth disease virus (FMDV) infection. Overexpression of miR-7139-3p attenuated FMDV replication, whereas inhibition promoted FMDV replication. In addition, the survival rate of FMDV infected suckling mice was increased through injection of miR-7139-3p agomiR. Further studies revealed that miR-7139-3p targets Bcl-2 to initiate the apoptotic pathway and caspase-3 cleaved 3Cpro behind the 174th aspartic acid (D174), which eventually promotes the degradation of 3Cpro. Overall, our findings demonstrate that miR-7139-3p suppresses FMDV replication by promoting degradation of 3Cpro through targeting the apoptosis-negative regulatory gene Bcl-2.PMID:38657363 | DOI:10.1016/j.virol.2024.110070
Source: Virology - Category: Virology Authors: Source Type: research